AF041808 5586 112 793 S. cerevisiae GAL10 promoter AF041808 5586 794 929 5' recombination region AF041808 5586 846 863 polyhistidine affinity tag AF041808 5586 867 899 T7 tag epitope AF041808 5586 912 926 enterokinase recognition sequence AF041808 5586 936 982 multiple cloning sites AF041808 5586 983 1188 3' recombination region AF041808 5586 1189 1649 ori bacteriophage f1 AF041808 5586 1708 2888 his3 AF041808 5586 3137 3513 S. cerevisiae ARSH4 AF041808 5586 3523 3651 cen6