AF041807 5973 112 793 S. cerevisiae GAL10 promoter AF041807 5973 794 929 5' recombination region AF041807 5973 846 863 polyhistidine affinity tag AF041807 5973 867 899 T7 tag epitope AF041807 5973 912 926 enterokinase recognition sequence AF041807 5973 926 981 multiple cloning sites AF041807 5973 973 1178 3' recombination region AF041807 5973 1238 2830 S. cerevisiae 2 micron plasmid STB and ARS region AF041807 5973 2854 3961 ura3