AF027127 4643 1 36 polylinker AF027127 4643 37 131 tk-promoter AF027127 4643 170 1822 luc+ AF027127 4643 1854 2075 splice and polyadenylation signals AF027127 4643 2078 2094 polylinker AF027127 4643 3277 4137 bla AF027127 4643 4269 4640 polyadenylation signals