CVU47121 3632 1 419 SV40 enhancer and early promoter CVU47121 3632 660 678 T7 promoter CVU47121 3632 678 730 multiple cloning sites CVU47121 3632 737 958 SV40 late polyadenylation signal CVU47121 3632 1048 1503 phage f1 region CVU47121 3632 1940 2800 ampR