AF031813 6748 15 1163 lacI AF031813 6748 15 46 lacI AF031813 6748 1406 1433 Ptac promoter AF031813 6748 1528 3018 malE/lacZ-alpha fusion AF031813 6748 2725 2836 malE/lacZ-alpha fusion AF031813 6748 3520 4380 ampR AF031813 6748 4422 4935 M13 origin of replication (+ -)