AY299696 5938 193 209 T7 promoter AY299696 5938 210 235 lac operator AY299696 5938 266 272 fusion protein start codon AY299696 5938 286 303 His-tag AY299696 5938 304 315 Factor Xa cleavage site AY299696 5938 370 393 FLAG-tag AY299696 5938 379 393 enterokinase cleavage site AY299696 5938 397 420 Strep-tag II AY299696 5938 487 534 T7 terminator AY299696 5938 1166 1828 pBBR1 oriV AY299696 5938 2822 3815 RP4 mob; oriT AY299696 5938 4280 5071 aadA AY299696 5938 5807 5922 T4 terminator