AY299693 5878 207 223 T7 promoter AY299693 5878 224 249 lac operator AY299693 5878 280 286 fusion protein start codon AY299693 5878 300 317 His-tag AY299693 5878 318 329 Factor Xa cleavage site AY299693 5878 441 488 T7 terminator AY299693 5878 1120 1782 pBBR1 oriV AY299693 5878 2776 3769 RP4 mob; oriT AY299693 5878 4234 5244 aadA AY299693 5878 5761 5876 T4 terminator