MK681508 5523 194 782 ColE1-like origin of replication MK681508 5523 974 992 promoter for bacteriophage T3 RNA polymerase MK681508 5523 1097 1107 stop codons in all the three reading frames MK681508 5523 1110 1121 optimized RBS MK681508 5523 1128 1844 gfpmut3 MK681508 5523 1864 1958 ble gene predicted promoter MK681508 5523 2149 2523 ble MK681508 5523 2905 3009 beta-lactamase promoter MK681508 5523 3027 3055 class 1 integron promoter MK681508 5523 3244 3777 aacC1 MK681508 5523 3937 3964 putative antitoxin-toxin module-predicted promoter