MK681507 7545 194 782 ColE1-like origin of replication MK681507 7545 974 992 promoter for bacteriophage T3 RNA polymerase MK681507 7545 1097 1107 stop codons in all the three reading frames MK681507 7545 1107 1117 optimized RBS MK681507 7545 1124 4198 lacZ MK681507 7545 4306 4334 ble gene predicted promoter MK681507 7545 4379 4753 ble MK681507 7545 4927 5031 beta-lactamase promoter MK681507 7545 5049 5077 class 1 integron promoter MK681507 7545 5266 5799 aacC1 MK681507 7545 5959 5986 putative antitoxin-toxin module-predicted promoter