EF216319 5813 120 935 aph EF216319 5813 1222 2009 derived from Escherichia coli; OriE EF216319 5813 2215 4799 mycobacterial origin; OriM EF216319 5813 4857 4943 BCG hsp60 EF216319 5813 4967 4973 strong synthetic Shine Delgarno sequence; RBSA EF216319 5813 4983 5699 gfp EF216319 5813 5734 5777 rrnBT1