AY070365 5261 9 75 multiple cloning site AY070365 5261 89 1543 lacI AY070365 5261 89 107 lacI AY070365 5261 116 144 lacP(A1/04/03) AY070365 5261 121 139 lacI AY070365 5261 1891 2082 rom AY070365 5261 2273 2274 inserted with Klenow polymerase to remove NdeI site AY070365 5261 2332 3107 RNA-DNA transition site AY070365 5261 3271 4131 bla AY070365 5261 4572 5027 phage f1 region AY070365 5261 5107 5197 T7 terminator region AY070365 5261 5245 5261 SP6 promoter