AY070364 4694 10 74 multiple cloning site; synthetic adapters AY070364 4694 58 1543 lacI AY070364 4694 58 64 lacI AY070364 4694 89 107 lacI AY070364 4694 121 139 lacI AY070364 4694 2704 3564 bla AY070364 4694 3949 3950 inserted with Klenow polymerase to remove NdeI site AY070364 4694 4005 4460 phage f1 region AY070364 4694 4540 4630 T7 terminator region AY070364 4694 4678 4694 SP6 promoter