LC217877 4729 1 263 pHSG299 HspTer LexAOpe 35SminPro LC217877 4729 14 263 hsp terminator LC217877 4729 276 1927 pENTR R4-pLac-Spect-R3 LC217877 4729 288 411 attR3 LC217877 4729 1779 1903 attR4 LC217877 4729 1940 4679 Gateway donor vector pDONR-Zeo LC217877 4729 1940 1965 attL2 LC217877 4729 2174 2479 ccdB LC217877 4729 3323 3996 pUC origin LC217877 4729 4643 4667 attL1 LC217877 4729 4680 4711 pHSG299 CSPS