CVU47119 4006 1 742 chimeric intron CVU47119 4006 1020 1041 T7-EEV sequencing primer binding site CVU47119 4006 1034 1052 T7 promoter CVU47119 4006 1052 1104 multiple cloning sites CVU47119 4006 1111 1332 SV40 late polyadenylation signal CVU47119 4006 1422 1877 phage f1 region CVU47119 4006 2314 3174 ampR