AY327138 4366 7 87 T5 AY327138 4366 115 171 Avi-tag AY327138 4366 172 456 bacteriophage lambda protein D AY327138 4366 457 474 6*His-tag AY327138 4366 475 519 multiple cloning site AY327138 4366 2489 3145 ColE1