AY327137 4361 7 87 T5 AY327137 4361 115 171 Avi-tag AY327137 4361 172 447 truncated N-terminus bacteriophage lambda protein D AY327137 4361 448 489 multiple cloning site AY327137 4361 490 510 6*His-tag AY327137 4361 2484 3140 ColE1